Quantification of polyphenols and flavonoid content and evaluation of anti-inflammatory and antimicrobial activities of Stenocereus stellatus extracts.

The hexanic, ethyl acetate and methanolic extracts from branches of Stenocereus stellatus were tested in both the 12-O-tetradecanoylphorbol-13-acetate (TPA) - induced ear oedema model and antimicrobial activity assay. The % of oedema inhibition, the Minimum Inhibitory Concentration (MIC), as well as the polyphenolic and flavonoid content were determined. Also, extracts were analysed by gas chromatography-mass spectrometry (GC-MS). In TPA model, the three extracts showed moderate oedema inhibition. In the antimicrobial activity assay, methanolic extract shows better MIC against all strains. The lowest MICs were for Candida albicans (31 µg/mL) and Rhizopus sp. (15 µg/mL). Also, 50.78 mg eq. of gallic acid/g extract of polyphenol and 115.12 mg eq. of catequine/g extract of flavonoids content were founded in ethyl acetate extract. In the chromatographic analysis, ß-sitosterol, ß-amyrine, betulin and some other molecules were identified. The results show that S. stellatus possess antimicrobial activities against some fungus species.

Espondilodiscitis tuberculosa: uso de los exámenes complementarios con una nueva perspectiva: A propósito de dos casos clínicos y revisión de la literatura / Tuberculous spondylodiscitis: use of complementary tests with a new perspective: A report of two cases and review of literature

Introducción. La tuberculosis constituye actualmente un grave problema sanitario. Es una enfermedad reemergente, su principal factor de riesgo es la infección por el virus de la inmunodeficiencia humana (VIH), siendo las formas extrapulmonares mucho más frecuentes en este grupo respecto a la población general. La espondilodiscitis tuberculosa (ET) representa 3% del total de las infecciones tuberculosas y 35% de las formas extrapulmonares. Su clínica es insidiosa, de diagnóstico complejo, la imagenología y microbiología son imprescindibles para un correcto diagnóstico. Todo esto suele determinar un retraso importante en el manejo, con consecuencias directas en el pronóstico del paciente. Material y método. Se presentan dos casos de ET asistidos en un Hospital público uruguayo (2012-2013), en pacientes con inmunocompromiso severo y noción de contacto epidemiológico en uno de ellos, diagnosticados tras la sospecha clínico-imagenológica y confirmación microbiológica por punción-aspiración bajo tomografía computarizada (TC). Resultados. Se inició el tratamiento con una latencia superior a tres meses. Discusión y Conclusiones. Las técnicas de biología molecular e inmunología constituyen hoy día una herramienta de gran valor para el diagnóstico precoz de esta enfermedad, permitiendo abreviar los tiempos en el inicio del tratamiento y reduciendo la tasa de complicaciones asociadas a ella.

Introduction. Tuberculosis (TB) constitutes a serious health problem nowadays. It is a reemerging disease whose main risk factor is the human immunodeficiency virus (HIV) infection, in which extrapulmonary forms are much more frequent than in general population. Tuberculous spondylodiscitis (TS) represents 3% of all TB infections and 35% of extrapulmonary forms. It has an insidious clinical presentation; the diagnosis is difficult requiring imagenologic and microbiologic technics. These characteristics result in a significant diagnosis delay which impacts on patient prognosis. Materials and methods. We present two cases of TS admitted to a public hospital in Uruguay (2012-2013) in immunocompromised patients and with epidemiological notion of contact in one of them. Results. The diagnosis was done after clinical and radiological suspicion; needle aspiration guided by computed tomography was performed. The treatment was instituted with a latency exceeding three months. Discussion and conclusions. The techniques of molecular biology and immunology are now a valuable tool for early diagnosis of this disease, shortening the initiation of treatment and reducing the rate of complications associated with it.

Consequences of cavity size and chemical environment on the adsorption properties of isoreticular metal-organic frameworks: an inverse gas chromatography study.

The role of the structure of three isoreticular metal-organic frameworks (IRMOFs) on their adsorption behavior has been studied in this work, selecting different kinds of volatile organic compounds (VOCs) as adsorbates (alkanes, alkenes, cycloalkanes, aromatics and chlorinated). For this purpose, three samples (IRMOF-1, IRMOF-8 and IRMOF-10) with cubic structure and without functionalities on the organic linkers were synthesized. Adsorption capacities at infinite dilution were derived from the adsorption isotherms, whereas thermodynamic properties have been determined from chromatographic retention volume. The capacity and the strength of adsorption were strongly influenced by the adsorbate size. This effect is especially relevant for n-alkanes adsorption, indicating the key role of the cavity size on this phenomenon, and hence the importance of the IRMOF structural properties. A different behavior has been observed for the polar compounds, where an enhancement on the specificity of the adsorption with the π-electron rich regions was observed. This fact suggests the specific interaction of these molecules with the organic linkers of the IRMOFs.

Experiencia de la clínica de traqueostomía del Hospital del Niños Doutor Carlos Sáenz Herrera, enero 2003-julio 2007 / Experience of the tracheostomy clinic of the national children Hospital Nacional de Niños Doutor Carlos Sáenz Herrera, January 2003 to July 2007

La traqueostomía ha sido realizada en un grupo grande de pacientes pediátricos, pero no hay estudios nacionales publicados. El objetivo de esta investigación es describir las características epidemiológicas y clínicas de la población traqueostomizada, en el periodo de estudio, para mejorar, con base en los hallazgos, las pautas generales de manejo. Materiales y métodos: Se efectuó una revisión retrospectiva de la población traqueostomizada desde 2003 hasta el primer semestre de 2007, para cuantificar la realización de la traqueostomías, historial de hospitalizaciones, uso de tratamiento profiláctico, historial de decanulaciones, decanulaciones fallidas, causa de muerte y clasificación técnica de la población atendida en la Clínica de Traqueostomía del Hospital Nacional de Niños "Dr. Carlos Sáenz Herrera". Se incluyeron 50 casos que acudieron al menos a una consulta durante el periodo de estudio, y se recopilaron los datos de edad, sexo, causa de realización de traqueostomía, complicaciones post traqueostomía, gérmenes causales de infección local, tratamiento, patologías asociadas, costos del equipo y materiales para el egreso. Resultados: En promedio se realizaron 28 traqueostomías anuales. El grupo etario al que se colocaron más traqueostomías fue de 1 a 3 años, con predominio en el sexo masculino (M: F,11:6); un 36 por ciento se efectuó a causa de parálisis cerebral infantil; se decanuló al 33 por ciento de los pacientes; hubo un 15 por ciento de decanulaciones fallidas. La mortalidad ocurrió en un 20 por ciento de los pacientes, por causas no relacionadas con la traqueostomía. El germen más frecuente causante de infección fue Pseudomona Aureoginosa (54 por ciento). El 22 por ciento de los pacientes con cultivos positivos utilizaron antibióticos profilácticos. Un 16 por ciento de los pacientes fue hospitalizado por problemas asociados con la traqueostomía...

The tracheostomy was performed in a large group of pediatric patients, but nopublished studies. The objective of this research is to describe the epidemiological and clinicalcharacteristics of the population traqueostomized during the study period, to improve based on the findings, the general pattern of management. Materials and methods: A retrospective review of the population traqueostomized since 2003to the first half of 2007 to quantify the performance of the tracheostomy, history of hospitalizations, use of prophylactic treatment, history of decanulations, decanulations failures, cause of death and the technical classification of the population attending the clinic of tracheostomy. We included 50 patients who attended at least one consultation during the study period and data were collected: age, sex, cause of realization of a tracheostomy, tracheostomy complications, germs that cause local infection, treatment, associated diseases, equipment costs and materials for release. Results: On average 28 tracheotomy were performed per year. The group that is ethereal tracheostomy was placed over 1 to 3 years, with predominance in males (M: F, 11:6), 36% for cerebral palsy child; 33% of patients are decanulation, there were 15% decanulations failures. Mortality occurred in 20% of patients, for reasons unrelated to the tracheostomy. The most common germ causing infection was Pseudomona Aureoginosa (54%); 22% of patients with positive cultures using prophylactic antibiotics; 16% of patients hospitalized for problems associated with tracheostomy. In 88% of cases the principal caregiver is the mother of which 72% were married, 56% had incomplete primary. The cost of equipment and materials necessary for the discharge from the hospital and home care was very high. Conclusions: The tracheostomy is a procedure common in pediatric practice in a specialized hospital, more and more useful for the handling of the complicated child that requires...

Cystic fibrosis mutations in Costa Rica.

Using polymerase chain reaction amplification of DNA in dried blood spots and a nonisotopic reverse dot blot hybridization method, we performed molecular genetic analysis for 6 and for 16 of the most common mutations of the cystic fibrosis transmembrane conductance regulator gene (CFTR) in 24 unrelated Costa Rican individuals with cystic fibrosis (CF). While many countries and ethnic groups have been surveyed for CF mutations since the cloning of CFTR, Costa Rica has not heretofore been studied. Moreover, Costa Rica represents an especially intriguing population because of its mixed European-African-Amerindian origins and the existence of a detailed historical record of the founding Spanish families. Thus, such a study may reveal not only the population frequencies of various mutant alleles in this country, but also something about their geographic migrations and ethnic founder effects. The most common CF mutation in Caucasians, deltaF508, was found in only 11 (23%) of the CF chromosomes studied, while the G542X mutation, relatively rare in the general population but more common in southern Europe, was observed in 12 (25%). None of the other mutations tested was found in any of the subjects. We failed to detect the second mutant allele in 17 subjects and could not detect either allele in 4 subjects. The high prevalence of the G542X mutation in our cohort, which exceeds that of both the general Caucasian population and the American Hispanic population, reflects the strong genetic influence of the original Spanish founding families of Costa Rica. These results highlight important differences in Costa Rican CF genotypes as compared both to other North American and European populations and to American Hispanics, raising important implications about isolated founder effects and strategies for population screening in that country.